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pcatenhancer  (New England Biolabs)


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    New England Biolabs pcatenhancer
    Pcatenhancer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3943 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcatenhancer/pm15722515-54-18-24?v=New+England+Biolabs
    Average 99 stars, based on 3943 article reviews
    pcatenhancer - by Bioz Stars, 2026-07
    99/100 stars

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    Cloning of the human BRCA2 gene silencer. (A) Sketch showing the promoter and the silencer regions in the context of the upstream sequence of human BRCA2 gene. (B) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATbasic and pCATenhancer constructs. (C) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATcontrol constructs having the silencer sequence. Results are means ± SE (n = 6).

    Journal: Biochemical and biophysical research communications

    Article Title: Identification and Characterization of a Transcriptional Silencer Upstream of the Human BRCA2 Gene

    doi: 10.1006/bbrc.1999.1652

    Figure Lengend Snippet: Cloning of the human BRCA2 gene silencer. (A) Sketch showing the promoter and the silencer regions in the context of the upstream sequence of human BRCA2 gene. (B) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATbasic and pCATenhancer constructs. (C) Relative CAT activity in cell extracts obtained from MCF-10F cells transfected with different pCATcontrol constructs having the silencer sequence. Results are means ± SE (n = 6).

    Article Snippet: Transformation of cells and reporter gene assay ( 4 – 6 ) The following plasmid vectors, procured from Promega (Madison, WI), were employed in our study: pCATbasic, pCATenhancer, pCATcontrol, pSV-b-galactosidase, pGL3-basic, pGL3-enhancer, pGL3-control and pRL-TK ( 5 ).

    Techniques: Cloning, Sequencing, Activity Assay, Transfection, Construct